Looks like not being able to assign a drive letter like they describe can happen if either it was formatted using a Mac in a format other than NTFS (which sounds like you didnt use a mac?) or it otherwise is a protected drive/partition.
Apparently converting the drive from GPT to MBR partition type can sometimes fix it. Here’s a similar thread where someone had the issue: https://www.reddit.com/r/techsupport/comments/9imgfj/cant_assign_drive_letter_to_hdd_option_is_greyed/
Just to be clear, have you restarted your PC? It could have disabled the port until restart.